[经验交流] 脂肪胺的高效液相色谱分离及质谱鉴定

脂肪胺的高效液相色谱分离及质谱鉴定
Determination of Aliphatic Amines by High Performance Liquid Chromatography with Fluorescence and Atmospheric Pressure Chemical Ionization Detection and Identification by Mass Spectrometry
出版物: 色谱CHINESE JOURNAL OF CHROMATOGRAPHY
Year:2005 Issue:06 Volume: 23

作者: 陈向明, 石运伟, 赵先恩, 张海峰, 尤进茂, CHEN Xiangming, SHI Yunwei, ZHAO Xianen, ZHANG Haifeng, YOU Jinmao, 
关键词: 高效液相色谱-质谱, 荧光检测, 柱前衍生, 脂肪胺, 

Determination of Aliphatic Amines by High Performance Liquid Chromatography with Fluorescence and Atmospheric Pressure Chemical Ionization Detection and Identification by Mass Spectrometry

文摘: 采用新型荧光试剂1,2-苯并-3,4-二氢咔唑-9-乙酸(BCAA)为柱前衍生化试剂,在Hypersil BDS-C18色谱柱上,通过梯度洗脱对12种游离脂肪胺进行了分离和在线质谱定性.以乙腈为溶剂,1-乙基-3-(3-二甲氨基丙基)环己碳二亚胺(EDAC)为缩合剂,在50 ℃条件下衍生反应15 min后获得稳定的荧光产物.激发波长和发射波长分别为333 nm和390 nm.采用大气压化学电离源(APCI)的正离子模式,实现了土壤和污水中脂肪胺的定性及其含量的测定.脂肪胺的线性相关系数大于0.999 3,检测限为12～28 fmol.

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