[资源分享] 安捷伦2007液相色谱高级研修班资料

这么好的东东是不是大家没有发现呀

我发现了,我下载了

Advantages of Using Sub-Two Micron Particles in HPLC

使用亚二微米颗粒在HPLC中的优势
Paul Barton

Outline概要

• Show how sub-2mm particle technology can be used to speed up LC analyses
亚二微米颗粒技术如何能用于加速液相色谱分析
• Understand how silica manufacture has been adapted to lower backpressure whilst maintaining efficiency
理解硅胶制作技术如何能够降低反压但同时保持柱效
• Understand the impact of using short, highly efficient, columns packed with sub-2mm particles, on system dwell volume and
extra-column effects
理解系统延迟体积和柱外效应对填装亚二微米颗粒的高效短柱的影响
•Understand the impact of using short, highly efficient, columns packed with sub-2mm particles, on data acquisition rate
理解采样速率对填装亚二微米颗粒高效短柱分离的影响

Conclusions 结论•

Short, efficient, sub-2mm particle columns can be used to dramatically speed up separations without sacrificing resolution
内装亚二微米颗粒的高效短柱可以显著加速分离，而不会牺牲分离度
• Innovative particle manufacture has resulted in robust 1.8mm Zorbax silica, which has significantly lower backpressure and the same high efficiency of competitor materials
革新的颗粒制作工艺可以生产出稳定耐用的1.8mm Zorbax硅胶。与对手公司相应填料相比，该填料反压明显低且具有相同的高效率
• When working with gradients, the gradient time, tG must be reduced in the same ratio as the column volumes, Vm
当使用梯度分离时，梯度洗脱时间tG必须以色谱柱体积Vm相同的比例变化(降低)
• Care must be taken to minimize extra-column effects and dwell volume as much as possible
必须注意将柱外效应和延迟体积的影响降至越小越好
• Narrow peak widths, generated by these columns need fast data acquisition rates to realise the benefits of sub-2mm particle columns
由小颗粒短柱产生的窄色谱峰宽需要快采集速率以获得亚二微米颗粒色谱柱的优势

A Guide To Speeding Up Your Separations
加速分离指南
Adam Woodhouse

Overview概要

•Choosing The Column
选择色谱柱
•Reducing The Column Length
降低色谱柱长
•Changing The Column Id
改变色谱柱内径
•Adjusting The Injection Volume
调整进样体积
•Transferring to 1.8um Columns
转换成1.8um色谱柱
•Speeding It Up
提速


Summary小结

• Shorter 1.8um columns allow faster analysis with same resolving power as longer 5um columns
1.8um的较短色谱柱可以与较长5um色谱柱保持相同的分离能力，但分析时间更短
• Change column diameter if necessary to allow for lower solvent consumption and higher accessible velocity
如需更低的溶剂消耗和更高的速度，可以改变色谱柱内径
• Adjust the injection volume to maintain the same mass loading on the smaller column
调整进样体积以保持小色谱柱上的相同载样量
• Adjust run time and gradient to allow for the shorter column
调整运行时间和梯度以适合小色谱柱分离
• Speed up the flow rate until max flow or max pressure or loss of resolution
提高流速直至流速或压力达到最大值或分离度降低

How Can Temperature Help
温度可怎样提供帮助
Adam Woodhouse, Agilent UK, Sept.2006
edited by An Rong, Agilent China, Dec.2006

Overview概要

•Affect of Temperature on Pressure
温度对压力的影响
•Speeding It Up
加速
•Thermal Stability
热稳定性
•Separation Robustness
分离稳定性/耐受性
•UV Sensitivity
紫外灵敏度
•LC/MS Sensitivity
液质联用灵敏度

Summary小结

• Use of elevated temperatures reduces viscosity of mobile phase and therefore backpressure
提高温度可以降低流动相粘度及其反压
• Higher linear velocities are achievable with reduced backpressure and therefore faster analysis times
随着反压的降低，可以实现更高的线速度，因而分析时间可以更短
• Selectivity can vary with Temperature allowing temperature to be manipulated to achieve better separations
选择性随温度变化，使得温度可以成为实现更好分离的选择性调节手段
• Using post column cooling drastically reduces the UV detector noise seen when a hot mobile phase cools in the flow cell
使用柱后冷却功能可以使热流动相在检测池中冷却，显著降低紫外检测器的噪音
• LC/MS sensitivity can be increased by using elevated temperatures into the MS
LC/MS灵敏度可以因液相色谱使用较高的温度而提高

Increasing Efficiency, Increasing Resolution -Better Separations
改善分离效率与分离度，获得更好的分离结果
Paul Barton

Outline概要

Sub-2mm particle columns can be used in two ways
亚二微米颗粒色谱柱可以根据需要按两种方式选用：
• Fast LC with short, highly efficient columns at conventional and high flow rates
用高效短柱在常规及高流速下进行快速液相色谱分析
• High Resolution LC with longer (100mm-150mm) columns and conventional flow rates
用较长(100mm-150mm)色谱柱在常规流速下进行高分离度液相色谱分析
• Longer (100mm – 150mm) sub-2mm particle columns have much higher system backpressure, but this is less of an issue because we are looking to exploit the efficiency gains, not speed gains – flow remains the same as for 5mm or 3mm column
较长亚二微米颗粒色谱柱会产生比较高的反压，但此时我们关心的是分离效率而不是速度－流速可以与5mm or 3mm 色谱柱保持相同

Conclusions 结论

• Longer (100mm and 150mm), sub-2mm particle columns can be used to significantly increase resolution
较长色谱柱的亚二微米色谱柱可以显著改善分离度
• Zorbax silica is manufactured the same way, regardless of particle size, ensuring no change in selectivity
无论粒径大小如何，Zorbax硅胶以相同方法制作，确保选择性无变化
• Using sub-2mm particle columns for complex samples can yield very high (GC-like) peak capacities
使用亚二微米色谱柱分离复杂样品可以获得很高的色谱峰容量(类似GC)
• Peak Capacity calculations may be used to estimate resolution gains for complex chromatograms
峰容量计算可用于估计复杂色谱图的分离度改善
• It is sometimes possible to realise BOTH speed and resolution gains using sub-2mm particle columns
使用亚二微米颗粒的色谱柱可能实现速度和分离度的同时改善

多谢共享

多谢了

好东西需要用心寻找。